Method of preparing solution enriched in xylanase using low molecular weight alcohol, organic salt and inorganic salt
US5320960A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Apr 3, 1992 |
| Grant date | Jun 14, 1994 |
| Priority date | — |
| Expiry date | Apr 3, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for preparing an aqueous solution enriched in xylanase from an aqueous mixture containing cellulase proteins and xylanase is disclosed. The method involves adding an amount of a low molecular weight alcohol to an aqueous mixture containing cellulase proteins and an organic salt so as to precipitate out the cellulase proteins other than EG III from the mixture. The method then involves adding an inorganic salt to the supernate produced in the previous step so as to form a second precipitate and a second supernate and then finally collecting the second precipitate from the second supernate to obtain a precipitate enriched in xylanase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.