Fluorometer detection system
US5323008A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 23, 1992 |
| Grant date | Jun 21, 1994 |
| Priority date | — |
| Expiry date | Mar 23, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6463
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An improved fluorescence detection system is provided which utilizes a relatively high powered, relatively high repetition rate light source with high speed detection electronics to increase system sensitivity and accuracy. In the preferred embodiments, a laser diode is the light source. In one embodiment, the position of a time window is varied to compile a decay profile of a fluorophore. In another embodiment, the time to detection of a photon is used to compile the decay profile. In one aspect of this invention, a histogram of the fluorescence decay is generated by determining a preliminary histogram of the shape and multiplying it by the ratio of the total number of events divided by the number of events comprising the preliminary histogram. In another aspect of this invention, the time of detection after excitation of the photon is started from a random time, such as after a preceding event is detected and the data stored.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.