Coupled transcription and translation in eukaryotic cell-free extract
US5324637A · kind A · utility
42Cited by
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76Claims
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Key dates
| Filing date | Nov 9, 1993 |
| Grant date | Jun 28, 1994 |
| Priority date | — |
| Expiry date | Nov 9, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for coupling transcription and translation from DNA using a solution comprising a eukaryotic cell-free extract comprising adding a sufficient amount of a magnesium compound to the extract to raise the magnesium concentration to a level where RNA is transcribed from DNA and RNA translates into protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.