Herpes virus thymidien kinase-encoding DNA
US5324664A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 1993 |
| Grant date | Jun 28, 1994 |
| Priority date | — |
| Expiry date | Jan 21, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/16743
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for isolating thymidine kinase-encoding DNA of a herpes virus are described. These methods utilize degenerate primers based on regions of relatively conserved amino acid sequence in herpes virus thymidine kinase proteins to initiate a polymerase chain reaction which yields large amounts of the thymidine kinase-encoding DNA. The methods are illustrated in the isolation of the thymidine kinase gene of feline herpes virus, which can be used to construct recombinant thymidine kinase-negative feline herpes viruses for purposes of constructing live vaccines and expression vectors. In addition, the regulatory elements of the feline herpes virus thymidine kinase gene are useful in the construction of recombinant DNA vectors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.