Capillary electrophoresis with tracking separation field
US5328578A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 15, 1993 |
| Grant date | Jul 12, 1994 |
| Priority date | — |
| Expiry date | Jun 15, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A capillary electrophoresis system utilizes a square "ring" capillary. The capillary has a hair-pin bend at each corner of the square. An opening in each bend permits fluid and electrical coupling with a respective electrolyte-containing vial. A distributor selectively routes the opposite terminals of a power supply to opposing corners of the square so that a sample component of interest migrates continuously downstream. A detector determines when the sample component traverses a detection point along the capillary. This detection is used to determine a migration rate for the sample component. When the sample component is estimated to be midway between corners, the distributor switches from applying a voltage differential between a second upstream corner and the downstream corner to applying a voltage differential between the upstream corner and the second downstream corner. This permits the sample component to migrate downstream past the downstream corner. The sample repeatedly traverses the ring until a reading from the detector determines that satisfactory resolution has been achieved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.