Methods of using labeled nucleotides
US5328824A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 8, 1987 |
| Grant date | Jul 12, 1994 |
| Priority date | — |
| Expiry date | Dec 8, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compounds having the structure: ##STR1## wherein B represents a purine, 7-deazapurine, or pyrimidine moiety covalently bonded to the C.sup.1' -position of the sugar moiety, provided that when B is purine or 7-deazapurine, it is attached at the N.sup.9 -position of the purine or 7-deazapurine and when B is pyrimidine, it is attached at the N.sup.1 -position; PA1 wherein A represents a moiety consisting of at least three carbon atoms which is capable of forming a detectable complex with a polypeptide when the compound is incorporated into a double-stranded ribonucleic acid, deoxyribonucleic acid duplex, or DNA-RNA hybrid; PA1 wherein the dotted line represents a chemical linkage joining B and A, provided that if B is purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine; and PA1 wherein each of x, y and z represents ##STR2## either directly, or when incorporated into oligo- and polynucleotides, provide probes which are widely useful. Applications include detection and localization of polynucleotide sequence…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.