Nucleic acid probe, test kit and diagnostic and purification methods
US5328825A · kind A · utility
22Cited by
6References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 6, 1992 |
| Grant date | Jul 12, 1994 |
| Priority date | — |
| Expiry date | Mar 6, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/703
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.