Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
US5328841A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 3, 1992 |
| Grant date | Jul 12, 1994 |
| Priority date | — |
| Expiry date | Apr 3, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/945
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for isolating EG III cellulase component and enriched EG III cellulase in polyethylene glycol are disclosed. The methods comprise adding an inorganic salt and polyethylene glycol having a molecular weight of from about 5,000 to 10,000 to an aqueous mixture of cellulase proteins under conditions to create a two-phase system. Next, the cellulase proteins other than EG III are separated in an EG III-poor aqueous phase while EG III cellulase component is retained in an EG III-rich polyethylene glycol phase. Lastly, the EG III component can be separated from the polyethylene glycol. The preferred method of separation is alcohol precipitation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.