Recombinant chimeric proteins deliverable across cellular membranes into cytosol of target cells

US5328984A · kind A · utility

Assignee

• The United States as represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Ira H. Pastan · Potomac, US
• Prior Trevor · Bethesda, US
• David Fitzgerald · Rockville, US
• Waldemar Debinski · Winston-Salem, US
• Clay B. Siegall · Cumberland, US

Key dates

• Filing date: Mar 4, 1991
• Grant date: Jul 12, 1994
• Expiry date: Mar 4, 2011

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Proteins that are impermeable and foreign to the eukaryotic cells can now be delivered across cellular membranes into the cytosol of target cells by making a chimeric protein having specific attributes. A method of making such chimeric proteins is disclosed. As an example, a chimeric protein PE-Bar with dual enzymatic activity has been made. The chimeric proteins can be used for cytotoxic, diagnostic or therapeutic purposes, such as for compensating the deficiency or defect of an enzyme or a protein which may be causative of a disease or an abnormality.