Method for mammalian cell transfection using 25-hydroxycholesterol
US5330904A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 17, 1992 |
| Grant date | Jul 19, 1994 |
| Priority date | — |
| Expiry date | Jun 17, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a process, a cell culture medium, and a kit for the rapid, high-efficiency transfection of mammalian cells with exogenous DNA. The process comprises incubating a cell culture in the presence of a transfection medium comprising a serum that is different from the serum in the normal growth medium used to grow the cells, and DNA to produce transfected cells. In a preferred embodiment the normal growth medium comprises fetal bovine serum and the transfection medium comprises a serum such as human, calf, horse, lamb, or pig serum. The transfection medium may further comprise an hydroxylated sterol such as 25-hydroxycholesterol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.