Apparatus and method for automated purification of extra-chromo-somal DNA from a cell suspension in a container
US5330914A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 26, 1990 |
| Grant date | Jul 19, 1994 |
| Priority date | — |
| Expiry date | Nov 26, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to an apparatus for automatic purification of extra-chromosomal DNA from a cell suspension in a container. The apparatus comprises a device for introducing the cell suspension from a container into a chamber; a device for agitating the contents of the chamber; a device for introducing a lysing solution to the chamber to lyse the cells therein; a device for introducing a precipitating solution into the chamber to precipitate chromosomal DNA and, optionally, proteins and cellular debris therein; a device for feeding the liquid contents of the chamber through a filter to a collecting system; a device for purifying extra-chromosomal DNA from the contents of the collecting device; a device for introducing a dissolving solution into the chamber to dissolve the precipitate remaining therein, and a device for feeding the dissolved precipitate to waste.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.