Process for liberating an analyte from its binding protein
US5332678A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jan 10, 1990 |
| Grant date | Jul 26, 1994 |
| Priority date | — |
| Expiry date | Jan 10, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process for the dissolving off of an analyte bound to a binding protein by breakdown of the binding protein using a cleaving agent which cleaves --S--S-- groups into SH groups in the alkaline range, wherein a 1,2-dithiolan-3-carboxylic acid of the formula: ##STR1## is used as the --S--S-- group cleaving agent in which n is a whole number of from 1 to 8. The process is used for preparing samples for analytical or investigational processes for the determination of a liberated analyte and especially for immunoassays for vitamins such as vitamin B.sub.12, steroids such as testosterone or for thyroid hormones such as T.sub.3 /T.sub.4.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.