Method for specific binding assays using a releasable ligand
US5332679A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 12, 1993 |
| Grant date | Jul 26, 1994 |
| Priority date | — |
| Expiry date | Feb 12, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54306
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Immunoassays and DNA probe assays utilizing a non-immune, reversible binding displacement system are provided. In the assay, a releasable ligand, a binding partner for the releasable ligand, an analyte of interest, an analytically detectable (reporter) group, and at least one binding partner for the analyte, are first attached to an insoluble phase so as to form reporter-labeled complex bound to an insoluble phase, followed by the addition of a displacer ligand which displaces the releasable ligand along with some portion of the reporter-labeled complex, so that the released reporter is analytically detectable in a free liquid medium and can be related to the concentration of analyte in the sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.