Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5332805A · kind A · utility
28Cited by
10References
10Claims
0Family size
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Key dates
| Filing date | Mar 29, 1991 |
| Grant date | Jul 26, 1994 |
| Priority date | — |
| Expiry date | Mar 29, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/6481
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The production of recombinant chymosin is disclosed in which an insoluble form of chymosin precursor is produced by a bacterial host cell transformed by a vector including a coding sequence for said precursor. Solubilization of said insoluble form of chymosin precursor is accomplished using urea at a concentration of at least 7M or guanidine hydrochloride at a concentration of at least 6M prior to cleaving said precursor to form chymosin. Said solubilization preferably additionally involves the denaturation of said precursor in an alkaline aqueous solution, e.g., at a pH between a 9 and 11.5
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.