Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
US5334296A · kind A · utility
37Cited by
6References
20Claims
0Family size
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Key dates
| Filing date | Jun 28, 1993 |
| Grant date | Aug 2, 1994 |
| Priority date | — |
| Expiry date | Jun 28, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytyes depending on the oxidase employed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.