Quantification of bacteria using a nucleic acid hybridization assay
US5334501A · kind A · utility
15Cited by
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19Claims
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Assignee
Inventors
Key dates
| Filing date | Apr 1, 1993 |
| Grant date | Aug 2, 1994 |
| Priority date | — |
| Expiry date | Apr 1, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides for a method of quantifying bacteria using a bacterial specific nucleic acid probe which is complementary to a unique and highly conserved region of the 16S ribosomal RNA (rRNA) of bacteria. This probe permits the rapid detection of 16S rRNA in a sample and by comparison with known standards, one can estimate the total bacterial count in the sample. The method is accurate and reproducible and conducted at temperatures of between about 120.degree. to about 40.degree. C.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.