Chromogenic method of detecting endoproteases

US5334506A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Werner Stuber · Lahntal, DE
• Dieter Schnaitmann · Eppstein, DE

Key dates

• Filing date: Oct 30, 1991
• Grant date: Aug 2, 1994
• Expiry date: Oct 30, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compounds of the formula I ##STR1## in which X denotes a hydrogen atom, a group which irreversibly masks the terminal amino group, or a protecting group which is conventional in peptide chemistry, such as, for example, Boc-, Z- or Fmoc-, PA1 A and B may be identical or different and denote an alpha- beta- or gamma-amino acid which comprises 2 to 15 carbon atoms and up to 4 nitrogen atoms, 2 sulfur atoms and 6 oxygen atoms and whose side chain may be substituted, and B, if appropriate, denotes a dipeptide formed from these amino acids, PA1 C denotes arginine, Lysine, tyrosine, phenylalanine or tryptophane, and their homologs, PA1 R.sub.1 and R.sub.2 are identical or different and denote a hydrogen atom or an alkyl radical having up to 4 carbon atoms, PA1 R.sub.3 to R.sub.8 are identical or different and denote hydrogen, an alkyl radical, an alkoxy radical or a halogen radical, PA1 Y denotes oxygen, and PA1 An.sup.- denotes an anion, and their water-soluble salts, and a process for their preparation are described. These compounds or salts can be used for detecting or determining enzymes.