Production of poly-beta-hydroxybutyrate in transformed escherichia coli
US5334520A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 25, 1990 |
| Grant date | Aug 2, 1994 |
| Priority date | — |
| Expiry date | May 25, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for enhancing the production of PHB from a transformed E. coli host which includes the genes coding for the PHB biosynthetic pathway. By inserting the genes coding for PHB into a host which includes a lactose utilization system, a low cost minimal medium including whey can be used as the fuel and carbon source for PHB production. A plasmid which codes for the PHB biosynthetic pathway plus four hundred extra bases on either side of the first and last genes in the pathway has been inserted into the host and has been shown to produce a larger amount of PHB accumulation in a shorter period of time than other plasmid constructs. CaCl.sub.2 has been shown to be an effective agglomerating agent for agglomerating PHB which has been produced in a transformed E. coli host.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.