Process for the preparation of optically pure diastereoisomers of tetrahydrofolate compounds using 10-formyltetrahydrofolate synthetase from clostridium
US5334535A · kind A · utility
4Cited by
2References
1Claims
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Key dates
| Filing date | Sep 18, 1992 |
| Grant date | Aug 2, 1994 |
| Priority date | — |
| Expiry date | Sep 18, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/842
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the preparation of optically pure diastereoisomers of tetrahydrofolate compounds is described, comprising the conversion, for example, of only the 5,6S,7,8-tetrahydrofolic acid component of a racemic mixture of 5,6,7,8-tetrahydrofolic acid to 10-formyl-5,6S,7,8-tetrahydrofolic acid in the presence of a formyl tetrahydrofolate synthetase, followed by cyclizing, hydrolyzing and derivatizing. The process is also useful to make a desired substantially pure (6R or 6S) enantiomer of a derivative of (radiolabeled) tetrahydrofolic acid or a salt or ester thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.