Expression enhancer and use thereof to increase the yield in the expression of recombinant genes

US5336602A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Ulrich Brinkmann · Weilheim, DE
• Ralf Mattes · Stuttgart, DE
• Peter Buckel · Bernried, DE

Key dates

• Filing date: Jan 22, 1993
• Grant date: Aug 9, 1994
• Expiry date: Jan 22, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An expression enhancer has a DNA sequence which is capable of forming a t-RNA clover-leaf structure after transcription and hybridizes in that region of the DNA which, after transcription, forms the anticodon loop in the clover-leaf structure with an oligonucleotide with the sequence 5'-GACTTAGAAGGTCGTT-3' or its complementary sequence (5'-AACGACCTTCTAAGTC-3'). It can be used to increase the yield in the expression of a recombinant gene by transformation of suitable host cells with an expression vector containing the recombinant gene, whereby it is likewise introduced into the host cells in a form capable of expression and expressed.