Heparinase free of an anticoagulant component from flavobacterium Heparinum
US5338677A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 15, 1993 |
| Grant date | Aug 16, 1994 |
| Priority date | — |
| Expiry date | Nov 15, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/85
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A heparinase derived from Flavobacterium heparinum which meets all requirements for a clinical reagent that can eliminate heparin interference of normal blood function has been developed. The heparinase, derived from Flavobacterium heparinum, is free of a component that inhibits coagulation wherein the anticoagulant component does not bind, and the heparinase does bind, to a polysulfated resin having a pH of 7.0 and a conductivity between 3 and 12 mmhos. It is stable under normal manufacturing, shipping and clinical storage conditions for at least one year. The heparinase in useful in vitro to eliminate the interference in hematological assays due to the presence of heparin. The heparinase is also useful for the in vivo neutralization of heparin during surgical procedures. Advantages of this enzyme are that it achieves neutralization faster and more completely than previously available enzymes and is stable for long periods of time.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.