Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces
US5338678A · kind A · utility
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Key dates
| Filing date | Jun 1, 1990 |
| Grant date | Aug 16, 1994 |
| Priority date | — |
| Expiry date | Jun 1, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/78
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Thermally stable cytosine deaminase (CDase), and the gene coding therefor, is disclosed as well as methods of isolating, purifying, and recombinantly producing the same. The thermally stable CDase can be isolated from Saccharomyces cerevisiae. The yeast isolated enzyme has a molecular weight of approximately 32 kDa, as determined by gel filtration chromatography, and is composed of two subunits, each with a molecular weight of about 17 kDa. Thermally stable yeast CDase so purified shows no significant sequence homology with other known sequenced proteins.
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