Method and card for detecting antigens and/or antibodies
US5338689A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 30, 1992 |
| Grant date | Aug 16, 1994 |
| Priority date | — |
| Expiry date | Oct 30, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/824
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of detecting target antibodies or antigens by reaction with specific binding partners thereto is disclosed. One of the target or the binding partner is bound to a carrier, and the other is unbound. The complex between the target and the binding partner, with one being carrier-bound, forms an optically detectable binding complex. A microreaction vessel having an upper portion, a transition portion and a lower portion is utilized, wherein the upper portion has a greater diameter or width than the lower portion, and the transition portion is situated between the upper portion and the lower portion, and is funnel shaped. The microreaction vessel contains a slurry or suspension of inert particles, and unbound target or binding partner thereto. A solution of the carrier bound target or binding partner thereto is added to the vessel, which is then centrifuged to produce an optical determination of the target. A strongly positive reaction is indicated by a complex lying upon or in a top portion of a layer inert particles, a weakly positive reaction is indicated by a complex located within a lower portion of the layer of inert particles, and a negative reaction is indicated by the …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.