Analysis of carbohydrates
US5340453A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 27, 1992 |
| Grant date | Aug 23, 1994 |
| Priority date | — |
| Expiry date | Mar 27, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/66
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Carbohydrate substances are separated or distinguished by a method which involves labelling carbohydrate substances with a labelling reagent comprising a fluorescent naphthalene ring structure having as a substituent a reactive group capable of reacting with a reducing sugar to bind thereto and also having at least one substituent group capable of carrying a charge but which does not react with reducing sugars and does not extinguish fluorescence of the labelling reagent; applying the labelled substances to an electrophoretic gel; and running the gel to cause differential migration of different substances. The preferred labelling reagents are aminoaphthalenesulphonic acids with one, two or three sulphonic acid groups, particularly 8-aminoaphthalene-1,3,6,-trisulphonic acid (ANTS), and 1-amino-4-naphthalene sulphonic acid (ANSA).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.