Patent · US Expired

Method for the determination of the concentration of an enzyme substrate and a sensor for carrying out the method

US5340722A · kind A · utility

640Cited by

5References

8Claims

0Family size

Assignee

AVL Medical Instruments · CH

Inventors

Otto S. Wolfbeis · Regensburg, DE
Wolfgang Trettnak · Mooskirchen, AT

Key dates

Filing date	Mar 29, 1993
Grant date	Aug 23, 1994
Priority date	—
Expiry date	Mar 29, 2013

Classification

Technology area (CPC G)Physics
CPC primaryG01N21/6428
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for the continuous and reversible determination of the concentration of an enzyme substrate such as glucose in an specimen, wherein the specimen is brought into contact with a corresponding enzyme selected from oxidases and oxygenases and to which a flavin coenzyme (FMN, FAD) is bonded, the flavin coenzyme changing to a reduced form by the enzyme substrate and to an oxidized form by molecular oxygen dissolved in the specimen, and the change in the fluorescence spectrum, produced by means of the reduction of the flavin coenzyme, is measured.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.