Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5340728A · kind A · utility
30Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 9, 1992 |
| Grant date | Aug 23, 1994 |
| Priority date | — |
| Expiry date | Dec 9, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method for performing a nested polymerase chain reaction (PCR) amplification of a targeted piece of DNA, wherein by controlling the annealing times and concentration of both the outer and the inner set of primers according to the method disclosed, highly specific and efficient amplification of a targeted piece of DNA can be achieved within one reaction vessel without depletion or removal of the outer primers from the reaction mixture vessel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.