Process for the recovery of recombinantly produced protein from insoluble aggregate
US5340926A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 5, 1993 |
| Grant date | Aug 23, 1994 |
| Priority date | — |
| Expiry date | Aug 5, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/1136
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In a process for the production of a soluble native protein, such as immunoglobulin or methionine-prochymosin, in which an insoluble form of the protein is produced by a host organism transformed with a vector including a gene coding for the protein, the insoluble form of the protein is reversibly denatured in an alkaline aqueous solution at a pH selected to promote dissociation of a group or groups of the protein involved in maintaining the conformation of the protein, and the protein is subsequently allowed to renature by reducing the pH of the solution below a pH effective to denature the protein to produce the soluble native form of the protein. The pH of the alkaline aqueous is suitably in the range 9.0 to 11.5.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.