Immobilization of thermostable microbial lipase by adsorption to macroporous inorganic carrier particles
US5342768A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 24, 1991 |
| Grant date | Aug 30, 1994 |
| Priority date | — |
| Expiry date | Apr 24, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A particulate immobilized microbial lipase is prepared by adsorption of lipase to macroporous inorganic carrier particles having specific characteristics. The carrier particles contain at least 65% silica or silicates, an excess of 90% particles having a size between 100 and 1000 .mu.m and an excess of 80% pores exhibiting a diameter of between 12 and 45 times the diameter of a lipase molecule. The lipase is adsorbed to the carrier particles by contacting the particles with an aqueous solution of lipase and drying the particles to a water content between 1 and 20%. The lipase is preferably a thermostable lipase from a Humicola species, Candida antarctica or Rhizomucor miehei. The particulate immobilized lipase is cheap to produce, exhibits a high specific activity, and can be used for interesterification of fats, hydrolysis of fats or synthesis of fatty acid esters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.