Process for enzyme immobilization on a fluorocarbon surface

US5342772A · kind A · utility

Assignees

• E.I. DU PONT DE NEMOURS AND COMPANY · US
• Baylor University · US

Inventors

• Rene Arenzen · Wilmington, US
• Patrick Boivin · Niort, FR
• Robert K. Kobos · Wilmington, US
• William H. Scouten · Providence, US
• Bruce E. Smart · Wilmington, US

Key dates

• Filing date: Feb 10, 1993
• Grant date: Aug 30, 1994
• Expiry date: Feb 10, 2013

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The fluorocarbon surface of a solid or liquid support is activated with a highly fluorinated isocyanate-modified ligand or with a reactive poly(fluoroalkyl) sugar reagent containing a polyhydroxy sugar to which are attached a plurality of fluoroalkyl anchor groups, a reactive group and optionally a spacer. The activated support has application in separation of biomolecules, immobilization of biomolecules, heterogeneous diagnostic assays, and biosensors. An enzyme or other biomolecule is immobilized by contacting the activated support surface with the enzyme in the presence of a surfactant. The surfactant is preferably a neutral surfactant such as a fluoroalkyl-polyoxyethylene.