Process for purifying DNA on hydrated silica
US5342931A · kind A · utility
44Cited by
10References
3Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 23, 1993 |
| Grant date | Aug 30, 1994 |
| Priority date | — |
| Expiry date | Apr 23, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for purifying DNA comprising 1) binding the DNA to a hydrated silica in the presence of water or physiological buffers in which the hydrated silica is prepared by refluxing silicon dioxide in sodium hydroxide or potassium hydroxide at a molar ratio of about 2:1 to 10:1 for at least about 48 hours, 2) separating and washing hydrated silica and the DNA bound thereto, and eluting the DNA from the hydrated silica in a heated physiological buffer or in heated water.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.