Gel electrophoresis measurement of dynamic reaction product by continuous sampling
US5344534A · kind A · utility
Inventor
Key dates
| Filing date | Mar 5, 1993 |
| Grant date | Sep 6, 1994 |
| Priority date | — |
| Expiry date | Mar 5, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/117497
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A kinetic reaction mixture is sampled continuously for determining the effect of a specific agent on the population density of a bio-organic molecule of interest as a function of time. A reaction sample containing the molecule of interest is processed through a high performance liquid chromatography column which produces longitudinal separation of a specific bio-organic molecule of interest. A continuous stream of longitudinally separated bio-organic molecules having a specific property of interest is delivered as a flowing stream into longitudinally separated sample receiving apertures formed in the face of a gel slab. Movement of the delivery nozzle and separation of the sample receiving apertures cause sample batches of reaction products to be deposited within separate channels, with the number of receiving channels corresponding generally with the number of discrete sample time intervals. The bio-organic molecules are clustered in discrete bands along the length of each receiver channel, with the vertical thickness of each band corresponding generally to the population density of the subject molecule during a specific time interval of the kinetic reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.