Process for the detection of nucleic acids

US5344757A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Hans-Joachim Holtke · Tutzing, DE
• Rudolf Seibl · Penzberg, DE
• Gudrun Schmitz · Deggendorf, DE
• Hans Scholer · Göttingen, DE
• Christoph Kessler · Munich, DE
• Ralf Mattes · Stuttgart, DE

Key dates

• Filing date: Jan 9, 1990
• Grant date: Sep 6, 1994
• Expiry date: Jan 9, 2010

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/686
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For the detection of nucleic acids of definite sequence by hybridisation with a complementary nucleic acid probe which contains bound via a chemical bonding at least one hapten as labelling one uses, as hapten, a steroid which is bound via a bridge of at least 4 atoms length to at least one position of the nucleic acid probe which does not participate in hydrogen bridge formation and detects the hybridised probe via an in turn labelled anti-hapten antibody.