Subtilisin modifications to enhance oxidative stability
US5346823A · kind A · utility
35Cited by
0References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 24, 1993 |
| Grant date | Sep 13, 1994 |
| Priority date | — |
| Expiry date | Mar 24, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/07
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
There are provided methods for making a mutant Bacillus subtilisin having altered oxidative stability, the methods comprising obtaining DNA fragment consisting of a region coding for a Bacillus subtilisin, and introducing a mutation into said DNA fragment such that the mutation is introduced in a region encoding a methionine, tryptophan, cysteine or lysine, sensitive to oxidation, such that upon expression of the mutant subtilisin one or more codon regions encoding for methionine, tryptophan, cysteine or lysine is replaced with an amino acid other than methionine, tryptophan, cysteine or lysine, preferably alanine or serine.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.