Process for isolating human albumin from supernatant IV, in particular IV-4, or from COHN's fraction V or from an analogous supernatant or fraction
US5346992A · kind A · utility
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4References
9Claims
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Key dates
| Filing date | Feb 6, 1992 |
| Grant date | Sep 13, 1994 |
| Priority date | — |
| Expiry date | Feb 6, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/765
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The process for isolating albumin from supernatant IV, and in particular IV-4, or from COHN's fraction V or from a plasma supernatant or fraction of analogous composition derived from an alcoholic or nonalcoholic fractionation, comprises one step on a hydrophilic anion exchange column with binding of albumin to the column, and then elution, and one step on a hydrophobic anion exchange column. Albumin thus obtained having a purity >99% by cellulose acetate electrophoresis, being free from impurities detected by crossed immunoelectrophoresis and free from polymers.
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