Patent · US Expired

Method for reducing non-specific priming in DNA amplification

US5348853A · kind A · utility

144Cited by
9References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 16, 1991
Grant dateSep 20, 1994
Priority date
Expiry dateDec 16, 2011

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6848
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention relates to a homogeneous process for amplifying a target sequence in a nucleic acid sample and detecting amplification in the absence of a separation step. The invention further provides a method for nucleic acid amplification under conditions which substantially reduce the occurrence of nonspecific amplification. Products and an apparatus related to the homogeneous process are also described.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.