Process for the enzymatic preparation of basic fibroblast growth factor
US5348863A · kind A · utility
4Cited by
4References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 2, 1992 |
| Grant date | Sep 20, 1994 |
| Priority date | — |
| Expiry date | Apr 2, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/503
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A bFGF is prepared by: (i) forming an adduct between heparin or heparan sulphate and a bFGF which has the 9-10 Leu-Pro bond; (ii) treating the adduct with pepsin A or cathepsin D, thereby cleaving the said bond; and (iii) releasing from the adduct the bFGF thus obtained. This process may be applied to prepare the 146 amino acid form of bFGF from longer forms of bFGF. It may also be used to produce a single form of bFGF from a mixture of bFGFs whose amino acid sequences differ only by having different N-terminii.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.