Purified luciferase from luciola lateralis
US5352598A · kind A · utility
19Cited by
1References
1Claims
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Assignee
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Key dates
| Filing date | Aug 29, 1991 |
| Grant date | Oct 4, 1994 |
| Priority date | — |
| Expiry date | Aug 29, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0.degree. C. to 50.degree. C., and that the enzyme does not act on ADP, CTP, UTP, and GTP. The enzyme is purified by using a process which includes: gel filtration chromatography, hydroxyapatite column chromatography, and a tris(hydroxy)aminomethane-hydro-chloric acid buffer.
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