Highly alkaline proteases

US5352603A · kind A · utility

Assignees

• Kali-Chemie Aktiengesellschaft · DE
• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Roman Vetter · Burgdorf, DE
• Detlef Wilke · Wrisbergholzen, DE
• Antoine Amory · Rixensart, BE
• Andre Clippe · Brussels, BE
• Dietmar Schomburg · Braunschweig, DE
• Wolfgang Aehle · Zwingenberg, DE

Key dates

• Filing date: Feb 28, 1991
• Grant date: Oct 4, 1994
• Expiry date: Feb 28, 2011

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/54
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.