Alkaline proteolytic enzyme and method of production

US5352604A · kind A · utility

Assignee

• Henkel Research Corporation · US

Inventors

• Charles R. Wilson · Santa Rosa, US
• Beth Frances Ladin · Santa Rosa, US
• Jonathan Richard Mielenz · Harriman, US
• Sherman S. M. Hom · Santa Rosa, US
• Dieter Hansen · Langenfeld, DE
• Robert B. Reynolds · Santa Rosa, US
• Nicholas C. T. Kennedy · Goldkronach, DE
• Joachim Schindler
• Michael Bahn · Hilden, DE
• Rolf Schmid · Gelterkinden, CH
• Martina Markgraf · Düsseldorf, DE
• Christian Paech · Santa Rosa, US
• Karlheinz Maurer · Erkrath, DE

Key dates

• Filing date: Mar 10, 1993
• Grant date: Oct 4, 1994
• Expiry date: Mar 10, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/75
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.