Method of type-specific detection of herpes simplex virus
US5354653A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 22, 1992 |
| Grant date | Oct 11, 1994 |
| Priority date | — |
| Expiry date | Oct 22, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/705
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method of the type-specific detection of herpes simplex virus, characterized by comprising the step of DNA amplification in a liquid mixture containing either of a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1a) ##STR1## with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2a): ##STR2## or a combination of a first DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (1b): ##STR3## with a second DNA primer containing an oligonucleotide having at least 15 bases in a base sequence of the formula (2b): ##STR4## a DNA polymerase and an aqueous liquid sample; and then the step of DNA examination on the resultant reaction solution. According to the present invention, it is possible to differentiate infectious disease caused by the HSV I or HSV II, accurately and rapidly in a type specific manner.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.