C-terminal amidating enzyme composition, process for preparing, and use of the same
US5354675A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 29, 1990 |
| Grant date | Oct 11, 1994 |
| Priority date | — |
| Expiry date | Jan 29, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/17003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A C-terminal amidating enzyme composition is disclosed having the ability to amidate the C-terminus of peptides with a C-terminal glycine. The C-terminal amidating enzyme is characterized by having a stable pH range of 5 to 9, metal ions and L-ascorbic acid as cofactors, two active fractions at molecular weights of about 50,000 and about 100,000 as determined from gel filtration, isoelectric points of about 4.5 pH for the active fraction having a molecular weight of about 50,000 and about 6.7 pH for the active fraction having a molecular weight of about 100,000 according to isoelectric point chromatography, and is activated by addition of catalase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.