Method for producing the DdeI restriction endonuclease and methylase
US5354680A · kind A · utility
2Cited by
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7Claims
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Key dates
| Filing date | Nov 26, 1991 |
| Grant date | Oct 11, 1994 |
| Priority date | — |
| Expiry date | Nov 26, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A two-step method for cloning the DdeI restriction modification system is provided which comprises introducing the DdeI modification or methylase gene into a host cell and expressing the gene to protect the DdeI host cell followed by introducing the DdeI restriction or endonuclese gene into the host.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.