Agrobacterium radiobacter K84 carrying transfer-deficient pAgK84 plasmids
US5354684A · kind A · utility
2Cited by
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4Claims
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Key dates
| Filing date | May 26, 1992 |
| Grant date | Oct 11, 1994 |
| Priority date | — |
| Expiry date | May 26, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides transfer-deficient pAgK84 plasmids, non-pathogenic strains of Agrobacterium radiobacter K84 carrying such plasmids, and a method useful for control of crown gall disease mediated by A. tumefaciens. The plasmids include genes encoding the synthesis of the antibiotic agrocin 84 and are modified in the transfer region of the plasmid by sufficient deletion to inhibit transfer of the plasmid. The plasmids are used to develop non-pathogenic strains of A. radiobacter K84 that stably produce agrocin 84 and inhibit the development of crown gall disease.
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