Assay for direct binding of peptides that are T-cell epitopes to MHC gene products on intact antigen-presenting cells and the use thereof for screening susceptibility of autoimmune diseases
US5356779A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 10, 1990 |
| Grant date | Oct 18, 1994 |
| Priority date | — |
| Expiry date | Dec 10, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
As assay for the measurement of direct binding of a peptide that is a T-cell epitope to gene products of the major histocompatibility complex (MHC), classes I and II, on the surface of intact living antigen-presenting cells, is provided. The assay comprises incubating the labelled peptide with the cells, and monitoring the extent of binding by the addition of a probe that reacts with the ligand used to label the peptide. The assay is suitable for autoimmune diseases and other immunological disorders. Peptides having a sequence corresponding to a stretch of the sequence of the antigen relevant to an immunological disorder, or modifications thereof, which bind to gene products of MHC, but do not stimulate T-cells, are also useful for the treatment of said disorders.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.