Determination of concentration by affinity titration
US5356784A · kind A · utility
17Cited by
4References
20Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Mar 30, 1993 |
| Grant date | Oct 18, 1994 |
| Priority date | — |
| Expiry date | Mar 30, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/97
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the amount of analyte in a sample utilizes a series of test regions with systematically varied, preferably monotonically increasing, affinity for a specific binding partner for an analyte or for the analyte itself. By determining the portion of test regions which are capable of binding specific binding partner in competition with the analyte in the sample, or which bind the analyte, the amount of analyte may be estimated. Thus, titration of affinity replaces titration of concentration, allowing assays to, be performed without the need for serial dilution steps.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.