Functional domains in flavobacterium okeanokoites (FokI) restriction endonuclease
US5356802A · kind A · utility
330Cited by
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6Claims
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Assignee
Inventor
Key dates
| Filing date | Apr 3, 1992 |
| Grant date | Oct 18, 1994 |
| Priority date | — |
| Expiry date | Apr 3, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present inventor have identified of the recognition and cleavage domains of the FokI restriction endonuclease. Accordingly, the present invention relates to DNA segments encoding the recognition and cleavage domains of the FokI restriction endonuclease, respectively. The 41 kDa N-terminal fragment constitutes the FokI recognition domain while the 25 kDa C-terminal fragment constitutes the FokI cleavage nuclease domain. The present invention also relates to hybrid restriction enzymes comprising the nuclease domain of the FokI restriction endonuclease linked to a recognition domain of another enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.