Sandwich immunoassay of .beta.-n-acetylglucosaminidase and monoclonal antibody used therein
US5358850A · kind A · utility
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11Claims
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Key dates
| Filing date | Jun 18, 1993 |
| Grant date | Oct 25, 1994 |
| Priority date | — |
| Expiry date | Jun 18, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a process of preparing a sandwich immunoassay of NAG, which comprises (1) reacting NAG with an immobilized anti-NAG monoclonal antibody and a labeled anti-NAG monoclonal antibody to form a complex of immobilized antibody-NAG-labeled antibody and (2) detecting the activity of said reacted and unreacted labeled anti-NAG antibody. This sandwich immunoassay is useful for diagnosis of renal disease, hepatitis, leukemia, and other such diseases. It also allows the direct and specific detection of NAG isozymes B and I in urine and blood.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.