Gene expression in mammalian cells using a cap independent 5' noncoding region
US5358856A · kind A · utility
7Cited by
1References
11Claims
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Key dates
| Filing date | May 18, 1992 |
| Grant date | Oct 25, 1994 |
| Priority date | — |
| Expiry date | May 18, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/203
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A construct which includes a cap independent 5' noncoding region of viral or cellular origin and a nucleotide sequence of interest, which is located downstream of the noncoding region. A method of producing a protein or a polypeptide of interest by introducing the construct, including a nucleotide sequence encoding the protein or the polypeptide of interest, into mammalian cells is also described. In one embodiment, the construct comprises all or a portion of the poliovirus cap independent 5' noncoding region and a nucleotide sequence encoding a protein or a polypeptide of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.