DNA sequencing
US5360523A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 17, 1993 |
| Grant date | Nov 1, 1994 |
| Priority date | — |
| Expiry date | Feb 17, 2013 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6417
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
To sequence DNA, DNA samples marked with fluorescent infrared dye are applied at a plurality of locations for electrophoresing in a plurality of channels through a gel electrophoresis slab. The channels are scanned with a laser and a sensor, that include a microscope focused on the gel slab. The focal point and slab are adjusted with respect to each other so that the focal point of the microscope remains on the gel slab during a scan. The data from the scan is directly used to amplitude modulate density readings on a display, and the scan is displayed in a horizontal sweep of a cathode ray tube, whereby said cathode ray tube provides intensity displays of bands representing DNA. Different sizes of glass gel sandwiches may be mounted to the same console for different sequencing tasks.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.