C-terminal .alpha.-amidating enzyme and process for production thereof
US5360727A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 3, 1992 |
| Grant date | Nov 1, 1994 |
| Priority date | — |
| Expiry date | Aug 3, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y114/17003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a C-terminal .alpha.-amidating enzyme of porcine origin having the following properties: (1) the action is on a peptide or protein represented by the formula: EQU X-R-Gly, wherein Gly represents a C-terminal glycine residue, R represents an amino acid residue to be .alpha.-amidated, and X represents a remaining portion of the peptide or protein to convert it to a peptide or protein represented by the formula: EQU X-R-NH.sub.2, wherein R-NH.sub.2 represents a C-terminal .alpha.-amidated amino acid residue and X represents a remaining portion of the peptide or protein; (2) the optimal pH is 6.5 to 8.5; (3) the molecular weight is about 92,000 as determined by SDS-polyacrylamide gel electrophoresis; and (4) it contains the following peptide fragment: EQU . . . Glu-Ala-Pro-Leu-Leu-Ile-Leu-Gly . . . . Further, the invention relates to a process for the production of the C-terminal .alpha.-amidating enzyme comprising the steps of extracting and purifying the enzyme from porcine atrium cordis exhibiting the enzyme activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.